CD93 anticorps

N° du produit ABIN111895

Détail du produit anti-CD93 anticorps

Antigène: CD93

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD93 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Purification: Affinity chromatography.

Immunogène: Human monocytes

Clone: X-2

Isotype: IgG1

Information d'application

Indications d'application: Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL IgG

Buffer: PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Ship at 2-8 °C.

Détails sur CD93

Antigène: CD93

Autre désignation: CD93 / C1QR1 (CD93 Produits)

Synonymes: anticorps 6030404G09Rik, anticorps AA145088, anticorps AA4.1, anticorps AW555904, anticorps C1qr1, anticorps C1qrp, anticorps Ly68, anticorps C1QR1, anticorps C1qR(P), anticorps C1qRP, anticorps CDw93, anticorps ECSM3, anticorps MXRA4, anticorps dJ737E23.1, anticorps C1qRp, anticorps CD93 antigen, anticorps CD93 molecule, anticorps Cd93, anticorps CD93

Sujet: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

ID gène: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3