ITGA5 anticorps (Biotin)

N° du produit ABIN111906

Cet anticorps anti-ITGA5 Monoclonal Souris conjugué à Biotin (Clone X-6) (ABIN111906) détecte spécifiquement ITGA5 dans FACS et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour ITGA5 anticorps (Biotin) (ABIN111906)

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA5 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-6

Détail du produit anti-ITGA5 anticorps (Biotin)

Specificité: This antibody X-6 is useful for the detection of the Fibronectin Receptor, or CD49e. Detects Human granulocytes, monocytes and platelets. Cross reactivity: unknown. X-6 detects an antigen of 154-180 kDa by Immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes, positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34+ cells, weak reaction on resting neutrophils. Staining increases with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes positive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to a decrease in the number of positive cells. Positive with cell lines U937, DH39 & MEP, HL-60, NB4, HMC-1. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.

Réactivité croisée (Details): Species reactivity (tested):Human: Granulocytes, Monocytes, Platelets.

Purification: Affinity Chromatography

Immunogène: Human Monocytes.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: use 10 μL stock solution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.01 % Thiomerosal as preservative and 10 mg/mL BSA as stabilizer.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Détails sur ITGA5

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Autre désignation: CD49e / ITGA5

Sujet: The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5

ID gène: 3678

NCBI Accession: NP_002196

UniProt: P08648

Pathways: Integrin Complex