CD46 anticorps

N° du produit ABIN111918

Cet anticorps anti-CD46 est un anticorps Souris Monoclonal détectant CD46 dans WB, FACS, IP et IHC (fro). Adapté pour Humain. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour CD46 anticorps (ABIN111918)

Antigène: CD46

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD46 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 13-42

Détail du produit anti-CD46 anticorps

Purification: Affinity Chromatography

Immunogène: Human U-251 cells.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry (IHC), FACS, Western Blots and Functional Studies. Recommended Dilutions: IHC on Frozen sections: 1-2 μg/mL (1/200-1/400)IHC on Paraffin sections: 8 μg/mL (1/50), Proteinase K pretreatment for antigen retrieval isrecommended. Flow Cytometry: 5-10 μg/mL of neat antibody to label 1x10^6 cells. Immunoprecipitation: 5 μg/ 200 μL extract from 2x10^6 cells. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Références pour anticorps anti-CD46 (ABIN111918)

Fett, Hermann, Muether, Kirchhof, Fauser: "Immunohistochemical localization of complement regulatory proteins in the human retina." dans: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).

Pedersen, Oster, Bundgaard, Höllsberg: "Induction of cell-cell fusion from without by human herpesvirus 6B." dans: Journal of virology, Vol. 80, Issue 19, pp. 9916-20, (2006) (PubMed).

Détails sur CD46

Antigène: CD46

Autre désignation: CD46 / MCP

Sujet: CD46 is also known as Membrane Cofactor Protein (MCP). CD46 acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.Synonyms: MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen

ID gène: 9606

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization