ICAM1 anticorps (Biotin)

N° du produit ABIN111935

L’anticorps Souris Monoclonal anti-ICAM1 a été validé pour WB, FACS, IHC (p) et IHC (fro). Il convient pour détecter ICAM1 dans des échantillons de Humain.

Aperçu rapide pour ICAM1 anticorps (Biotin) (ABIN111935)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1304

Détail du produit anti-ICAM1 anticorps (Biotin)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogène: Cultured Human endothelial cells

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 1 % BSA as stabilizer.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE.

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: CD54 / ICAM1

Sujet: ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

ID gène: 9606

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling