Endothelial Cells anticorps

N° du produit ABIN111945

Cet anticorps anti-Endothelial Cells est un anticorps Rat Monoclonal détectant Endothelial Cells dans IHC (fro). Adapté pour Humain.

Aperçu rapide pour Endothelial Cells anticorps (ABIN111945)

Antigène: Endothelial Cells

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Endothelial Cells est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1F10

Détail du produit anti-Endothelial Cells anticorps

Purification: Affinity A chromatography.

Immunogène: Cultured HUVECs.

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Frozen sections: 2 μg/mL (1: 100),Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigenexpression on cultured HUVEC is dependent on the culture conditions. Best results wereobtained (40-70 % pos. ) by daily renewal of FCS containing medium supplemented withendothelial cell conditioned supernatant whereas commercial available specificendothelial cell media have no influence on the antigen expression (see Table 1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Détails sur Endothelial Cells

Antigène: Endothelial Cells

Sujet: Synonyms: Endothelial Cell marker