GFAP anticorps

N° du produit ABIN111958

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, IF, IHC (p) et IP. Il présente une réactivité envers Humain, Porc et Cat.

Aperçu rapide pour GFAP anticorps (ABIN111958)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Porc, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: GF-01

Détail du produit anti-GFAP anticorps

Specificité: The antibody reacts with GFAP molecules. GFAP is the principal marker of astroglial cells in the central nervous system, it is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.

Purification: Protein-A Affinity Chromatography

Pureté: > 95 % (by SDS-PAGE).

Immunogène: Pellet of Porcine brain cold-stable proteins after depolymerization of microtubules.

Isotype: IgG2b

Information d'application

Indications d'application: Western Blotting. Immunoprecipitation. Immunocytochemistry: 5-10 μg/mL. Immunohistochemistry on Paraffin Sections: 10 μg/mL. The antibody strongly stains astrocytes in Human brain tissue sections but it is essentiallynegative on Mouse and Rat tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C longer.

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Glial Fibrillary Acidic Protein (GFAP) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier). In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimers disease, epilepsy and multiple sclerosis. Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.Synonyms: Glial Fibrillary Acidic Protein

ID gène: 2670

NCBI Accession: NP_001229305

UniProt: P14136