SFTPD anticorps (Conformational Domain)

N° du produit ABIN111974

Détail du produit anti-SFTPD anticorps

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Épitope: Conformational Domain

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SFTPD est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: SPDE recognizes a conformational epitope of SP-D. Clone SPDE is a reclone from clone IIE11, with identical reactivity. Antigen Distribution on Tissue Sections: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

Purification: Affinity Chromatography.

Immunogène: Purified Rat Surfactant protein D from bronchoalveolar lavage.

Clone: IIE11 (SPDE)

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 2 μg/mL (1/250)Does not react on routinely processed paraffin sections. Recommneded Positive Control: Rat lung. Has been described to work in Western blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water (= 0.5 mg/mL Stock Solution).

Concentration: 0.5 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for up to one month. For long term storage, aliquot and freeze unused portion at -20 °C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.

Références pour anticorps anti-SFTPD (ABIN111974)

Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." dans: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

Détails sur SFTPD

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SFTPD (SFTPD Produits)

Synonymes: anticorps COLEC7, anticorps PSP-D, anticorps SFTP4, anticorps SP-D, anticorps BDE, anticorps BDSD, anticorps HOX4I, anticorps SPD, anticorps AI573415, anticorps Sftp4, anticorps surfactant protein D, anticorps homeobox D13, anticorps surfactant associated protein D, anticorps SFTPD, anticorps HOXD13, anticorps Sftpd, anticorps SP-D

Sujet: Surfactant Protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

ID gène: 6441

NCBI Accession: NP_003010

UniProt: P35247