SFTPD anticorps (Conformational Domain) (Biotin)

N° du produit ABIN111975

Cet anticorps anti-SFTPD est un anticorps Souris Monoclonal détectant SFTPD dans WB, IHC (p) et IHC (fro). Adapté pour Humain et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour SFTPD anticorps (Conformational Domain) (Biotin) (ABIN111975)

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SFTPD est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: IIE11

Détail du produit anti-SFTPD anticorps (Biotin)

Épitope: Conformational Domain

Specificité: II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A. Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

Immunogène: Purified rat surfactant protein D from bronchoalveolar lavage

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on frozen sections (1 μg/mL, 1: 400). Immunohistochemistry on paraffin sections (1: 100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W). Western Blot. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline pH 7.2, (PBS), 5 mg/mL bovine serum albumin (BSA) as a stabilizer, no preservative.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store (in aliquots) at -20 °C for longer.

Références pour anticorps anti-SFTPD (Biotin) (ABIN111975)

Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." dans: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

Détails sur SFTPD

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SFTPD

Sujet: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

ID gène: 25350

NCBI Accession: NP_037010

UniProt: P35248