SFTPD anticorps (Conformational Domain) (Biotin)

N° du produit ABIN111975

Détail du produit anti-SFTPD anticorps (Biotin)

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Épitope: Conformational Domain

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SFTPD est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A. Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

Immunogène: Purified rat surfactant protein D from bronchoalveolar lavage

Clone: IIE11

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on frozen sections (1 μg/mL, 1: 400). Immunohistochemistry on paraffin sections (1: 100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W). Western Blot. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore by adding 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Phosphate buffered saline pH 7.2, (PBS), 5 mg/mL bovine serum albumin (BSA) as a stabilizer, no preservative.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store (in aliquots) at -20 °C for longer.

Références pour anticorps anti-SFTPD (Biotin) (ABIN111975)

Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." dans: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

Détails sur SFTPD

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SFTPD (SFTPD Produits)

Synonymes: anticorps COLEC7, anticorps PSP-D, anticorps SFTP4, anticorps SP-D, anticorps BDE, anticorps BDSD, anticorps HOX4I, anticorps SPD, anticorps AI573415, anticorps Sftp4, anticorps surfactant protein D, anticorps homeobox D13, anticorps surfactant associated protein D, anticorps SFTPD, anticorps HOXD13, anticorps Sftpd, anticorps SP-D

Sujet: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

ID gène: 25350

NCBI Accession: NP_037010

UniProt: P35248