MHC Class II Antigen I Ak,d,b,q,r anticorps (Biotin)

N° du produit ABIN111988

Détail du produit

Antigène: MHC Class II Antigen I Ak,d,b,q,r

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aucune reactivité croisée: Humain

Réactivité croisée (Details): Species reactivity (tested):Mouse (Cells expressing MHC class II antigens).

Purification: Affinity Chromatography

Immunogène: Murine thymic reticulum Remarks: Antigen / Epitope: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34 kDa) and one beta-chain (26-29 kDa). The epitope has not been further characterized.

Clone: ER-TR3

Isotype: IgG2b

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Suggested Positive Control: Mouse spleen. Does not react on routinely processed paraffin sections. Has been reported to work in Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock Solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer with no preservative

Agent conservateur: Without preservative

Conseil sur la manipulation: This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C

Stockage commentaire: Store at +4 °C. DO NOT FREEZE. This product should be stored undiluted.

Détail du antigène

Antigène: MHC Class II Antigen I Ak,d,b,q,r

Autre désignation: MHC Class II (I-A K,b,d,q,r)

Sujet: MHC Class II antigens are heterodimers consisting of one alpha chain (31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR3, ER-TR2, ER-TR1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the Human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.