Soluble Terminal Complement Complex (sC5b-9) anticorps

N° du produit ABIN112002

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans FACS, IHC (fro) et IHC (p). Adapté pour Humain.

Aperçu rapide pour Soluble Terminal Complement Complex (sC5b-9) anticorps (ABIN112002)

Antigène: Soluble Terminal Complement Complex (sC5b-9)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: X-11

Détail du produit

Réactivité croisée (Details): Species reactivity (tested):Human: Follicular dendritic (reticulum) cells, C9 neoepitope.No cross reaction observed when tested with polymerized complement C9 of 38 different species, including Mouse, Rat, Rabbit and Guinea Pig.

Purification: Affinity Chromatography

Immunogène: Enriched Human blood dendritic cells. Remarks: The antigen is a neoepitope of C9 after assembly in the terminal complement complex. Epitope sequence has not been determined.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 0.4 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/200). Microwave pretreatment forantigen retrieval is recommended. Has been described ro work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Place slide in a cuvette with 250ml 0. 01M citrate buffer, pH 6. 03. Heat slide in a microwave oven for 2 x 7min. at 700Watt4. Leave slide in the buffer for 20min for cooling5. Wash in distilled water6. Block endogenous peroxidase7. Wash in PBS8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber10. Wash in PBS11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber12. Wash in PBS13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 0.1 % Kathon, 5 mg/mL BSA

Agent conservateur: Kathon CG

Précaution d'utilisation: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détail du antigène

Antigène: Soluble Terminal Complement Complex (sC5b-9)

Autre désignation: Complement SC5b-9 (TCC / MAC)

Sujet: Follicular dendritic cells and sinus lining cells in lymph node and tonsil, and blood dendritic cells express the X-11 antigen. X-11+ blood dendritic cells show typical dendritic veils and are the most potent stimulator cells in an allogenic mixed leukocyte reaction when compared with other leukocytes. X11+ cells strongly react with anti S100 and weakly with anti CD68. The literature suggests that X-11+ cells are dendritic cells of monocytic origin.Synonyms: Marker for Complement C9 Neoepitope On Follicular Dendritic Cells, Membrane attack complex, Terminal complement complex