Desmoglein 2 anticorps

N° du produit ABIN112136

L’anticorps Souris Monoclonal anti-Desmoglein 2 a été validé pour WB, IF, IHC (p), IHC (fro) et EIA. Il convient pour détecter Desmoglein 2 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Desmoglein 2 anticorps (ABIN112136)

Antigène: Desmoglein 2 (DSG2)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmoglein 2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 10G11

Détail du produit anti-Desmoglein 2 anticorps

Specificité: The antibody recognizes Desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide Reacting: Desmoglein 2 (1069aa, calculated Mol. Wt. 116,760). Reactivity on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7. Negative with Mouse and Rat.

Purification: Affinity Chromatography on Protein A

Immunogène: Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Frozen Sections: For better resolution preincubation (directlyafter fixation) with 0.05-0.2 % Triton X-100 for 5-10 min, depending on tissue type, isrecommended (see Ref.3). Immunohistochemistry on Paraffin Sections: Use microwave treatment. Incubation Time: 1 h at RT for Frozen Tissue and overnight at 2-8 °C for Paraffin Sections. Working Dilution: Dilute 1/10 with PBS, pH 7.4 for Immunohistochemical applications . Recommended Positive Control: CaCo-2, HaCaT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL distilled water

Buffer: Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Références pour anticorps anti-Desmoglein 2 (ABIN112136)

Schlegel, Meir, Heupel, Holthöfer, Leube, Waschke: "Desmoglein 2-mediated adhesion is required for intestinal epithelial barrier integrity." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 298, Issue 5, pp. G774-83, (2010) (PubMed).

Détails sur Desmoglein 2

Antigène: Desmoglein 2 (DSG2)

Autre désignation: Desmoglein-2

Sujet: Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.Synonyms: CDHF5, Cadherin family member 5, DSG2, HDGC

ID gène: 1829

NCBI Accession: NP_001934

UniProt: Q14126

Pathways: Cell-Cell Junction Organization