ACTC1 anticorps

N° du produit ABIN112213

Cet anticorps Souris Monoclonal détecte spécifiquement ACTC1 dans WB, IHC (p), IHC (fro) et EIA. Il présente une réactivité envers Humain, Lapin, Boeuf (Vache) et Poulet et a été mentionné dans 3+ publications.

Aperçu rapide pour ACTC1 anticorps (ABIN112213)

Antigène: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivité: Humain, Lapin, Boeuf (Vache), Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: AC1-20-4-2

Détail du produit anti-ACTC1 anticorps

Specificité: This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Rabbit and Chicken.

Purification: Affinity Chromatographyon Protein G.

Immunogène: Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.

Isotype: IgG1

Information d'application

Indications d'application: ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1.0 mL distilled water.

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-ACTC1 (ABIN112213)

Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." dans: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

Itzhaki-Alfia, Leor, Raanani, Sternik, Spiegelstein, Netser, Holbova, Pevsner-Fischer, Lavee, Barbash: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." dans: Circulation, Vol. 120, Issue 25, pp. 2559-66, (2009) (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." dans: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

Détails sur ACTC1

Antigène: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Sujet: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.

ID gène: 70

NCBI Accession: NP_005150

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction