ACTC1 anticorps (Actin, Alpha, Cardiac Muscle 1)

Details for Product anti-ACTC1 Antibody No. ABIN112213
Antigène
  • acta1
  • actc
  • fa27h01
  • fb83f06
  • wu:fa27h01
  • wu:fb83f06
  • ACTC
  • ASD5
  • CMD1R
  • CMH11
  • LVNC4
  • Actc-1
  • cmd1r
  • ACTC1
  • actin, alpha, cardiac muscle 1 L homeolog
  • actin, alpha, cardiac muscle 1b
  • actin, alpha, cardiac muscle 1
  • actc1.L
  • actc1b
  • ACTC1
  • Actc1
  • actc1
Reactivité
Poulet, Boeuf (Vache), Humain, Lapin
59
42
36
9
6
4
4
4
2
Hôte
Souris
41
21
2
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp ACTC1 est non-conjugé
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
22
19
10
6
5
5
4
4
2
Options
Immunogène Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.
Clone AC1-20-4-2
Isotype IgG1
Specificité This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).
Réactivité croisée (Details) Species reactivity (tested):Human, Bovine, Rabbit and Chicken.
Purification Affinity Chromatographyon Protein G.
Plasmids, Primers & others Plasmids, Primers & others ACTC1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Sujet Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
ID gène 70
NCBI Accession NP_005150
UniProt P68032
Pathways Myometrial Relaxation and Contraction
Indications d'application ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1.0 mL distilled water.
Buffer Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Stock 4 °C/-20 °C
Stockage commentaire Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Produit citée dans: Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." dans: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, 2009 (PubMed).

Itzhaki-Alfia, Leor, Raanani, Sternik, Spiegelstein, Netser, Holbova, Pevsner-Fischer, Lavee, Barbash: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." dans: Circulation, Vol. 120, Issue 25, pp. 2559-66, 2009 (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." dans: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, 2007 (PubMed).

Background publications Moll, Holzhausen, Mennel, Kuhn, Baumann, Taege, Franke: "The cardiac isoform of alpha-actin in regenerating and atrophic skeletal muscle, myopathies and rhabdomyomatous tumors: an immunohistochemical study using monoclonal antibodies." dans: Virchows Archiv : an international journal of pathology, Vol. 449, Issue 2, pp. 175-91, 2006 (PubMed).

Ehler, Fowler, Perriard: "Myofibrillogenesis in the developing chicken heart: role of actin isoforms and of the pointed end actin capping protein tropomodulin during thin filament assembly." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 229, Issue 4, pp. 745-55, 2004 (PubMed).

Rudy, Yatskievych, Antin, Gregorio: "Assembly of thick, thin, and titin filaments in chick precardiac explants." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 1, pp. 61-71, 2001 (PubMed).

Franke, Stehr, Stumpp, Kuhn, Heid, Rackwitz, Schnoelzer, Baumann, Holzhausen, Moll: "Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells." dans: Differentiation, research in biological diversity, Vol. 60, Issue 4, pp. 245-50, 1996 (PubMed).

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