Desmocollin 3 anticorps (DSC3)

Details for Product anti-DSC3 Antibody No. ABIN112239, Fournisseur: Connectez-vous pour afficher
Antigène
  • CDHF3
  • DSC
  • DSC1
  • DSC2
  • DSC4
  • HT-CP
  • 5430426I24Rik
  • MGC85083
  • desmocollin 3
  • desmocollin 3 S homeolog
  • DSC3
  • Dsc3
  • dsc3.S
  • dsc3
Reactivité
Humain
63
32
17
2
1
1
1
1
Hôte
Souris
49
14
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp Desmocollin 3 est non-conjugé
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
24
14
13
9
3
3
2
2
2
1
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of Human Desmocollin 3.
Séquence VHGAPFYFSL PNTSPEISRL WSLTKVC
Clone Dsc3-U114
Isotype IgG1
Specificité This anibody localizes Dsc3 present in: - all living epidermal layers - in glandular duct cells - in basal matrix cells and outer root sheath of hair follicles - basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea - thymic reticulum cells All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most tested cultured cell lines derivated from stratified squamous epithelia or squamous cell carcinoma are positive (e.g A-431, HaCat). Does not react with Bovine and Rodent.
Purification Affinity Chromatography on Protein A
Plasmids, Primers & others Plasmids, Primers & others Desmocollin 3 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Desmocollin-3 (DSC3 Antibody Extrait)
Sujet Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.Synonyms: CDHF3, Cadherin family member 3, DSC3, DSC4, Desmocollin-4, HT-CP
ID gène 1825
NCBI Accession NP_001932
UniProt Q14574
Indications d'application Immunoblotting (Western). Immunohistochemistry on Frozen Sctions. Immunohistochemistry on Paraffin-Embedded Sections after microwave treatment: 1/10 inPBS ( pH 7.4) for 1h at RT. Recommendations: For better resolution on Frozen Sections preincubation (directly afterfixation) with 0.05-0.2 % Triton X100, for 5-10 min, depending on tissue type, isrecommended (Ref.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL distilled water.
Buffer PBS buffer, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation This product is photosensitive and should be protected from light
Stock 4 °C
Stockage commentaire Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Produit citée dans: Lorén, Cabré, Ojanguren, Domènech, Pedrosa, García-Jaraquemada, Mañosa, Manyé et al.: "Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in ..." dans: PLoS ONE, Vol. 10, Issue 6, pp. e0130921, 2016 (PubMed).

Brenner, Riha, Giessrigl, Thalhammer, Grusch, Krupitza, Stieger, Jäger: "The effect of organic anion-transporting polypeptides 1B1, 1B3 and 2B1 on the antitumor activity of flavopiridol in breast cancer cells." dans: International journal of oncology, Vol. 46, Issue 1, pp. 324-32, 2015 (PubMed).

Hamidov, Altendorf-Hofmann, Chen, Settmacher, Petersen, Knösel: "Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma." dans: Journal of clinical pathology, Vol. 64, Issue 11, pp. 990-4, 2012 (PubMed).

Cui, Chen, Yang, Mireskandari, Knösel, Zhang, Kohler, Kunze, Presselt, Petersen: "Diagnostic and prognostic impact of desmocollins in human lung cancer." dans: Journal of clinical pathology, Vol. 65, Issue 12, pp. 1100-6, 2012 (PubMed).

Goris, Uhlenbruck, Schwegmann-Wessels, Köhl, Niedorf, Stern, Hewicker-Trautwein, Bals, Taylor, Braun, Bicker, Kietzmann, Herrler: "Differential sensitivity of differentiated epithelial cells to respiratory viruses reveals different viral strategies of host infection." dans: Journal of virology, Vol. 83, Issue 4, pp. 1962-8, 2009 (PubMed).

Background publications Akat, Mennel, Kremer, Gassler, Bleck, Kartenbeck: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." dans: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003 (PubMed).

South, Wan, Stone, Dopping-Hepenstal, Purkis, Marshall, Leigh, Eady, Hart, McGrath: "Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability." dans: Journal of cell science, Vol. 116, Issue Pt 16, pp. 3303-14, 2003 (PubMed).

Hatzfeld, Haffner, Schulze, Vinzens: "The function of plakophilin 1 in desmosome assembly and actin filament organization." dans: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000 (PubMed).

Moll, Houdek, Schäfer, Nuber, Moll: "Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model." dans: Archives of dermatological research, Vol. 291, Issue 7-8, pp. 437-46, 1999 (PubMed).

Kurzen, Moll, Moll, Schäfer, Simics, Amagai, Wheelock, Franke: "Compositionally different desmosomes in the various compartments of the human hair follicle." dans: Differentiation; research in biological diversity, Vol. 63, Issue 5, pp. 295-304, 1998 (PubMed).

Nuber, Schäfer, Stehr, Rackwitz, Franke: "Patterns of desmocollin synthesis in human epithelia: immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells." dans: European journal of cell biology, Vol. 71, Issue 1, pp. 1-13, 1997 (PubMed).

Schäfer, Stumpp, Franke: "Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues." dans: Differentiation; research in biological diversity, Vol. 60, Issue 2, pp. 99-108, 1996 (PubMed).

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