Uroplakin 3A anticorps

N° du produit ABIN112250

L’anticorps Souris Monoclonal anti-Uroplakin 3A a été validé pour WB et IHC (p). Il convient pour détecter Uroplakin 3A dans des échantillons de Humain, Rat, Boeuf (Vache) et Porc.

Aperçu rapide pour Uroplakin 3A anticorps (ABIN112250)

Antigène: Uroplakin 3A (UPK3A)

Reactivité: Humain, Rat, Boeuf (Vache), Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Uroplakin 3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AU1

Détail du produit anti-Uroplakin 3A anticorps

Specificité: Clone AU1 reacts specifically with Uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60 % of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of Uroplakin III. Until now, no Uroplakin staining was found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Pig and Rat.

Purification: Affinity Chromatography on Protein A.

Immunogène: Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting (Western blotting). Immunohistochemistry on Paraffin-Embedded Tissue (not on Frozen Sections! ) using aheat-induced retrieval method (e. g. microwave treatment): Working dilution: 1/10. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: Final solution contains PBS, pH 7.4 with 0.5 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur Uroplakin 3A

Antigène: Uroplakin 3A (UPK3A)

Autre désignation: Uroplakin-3a (UPK3A)

Sujet: Together with the Uroplakins UP Ia, UP Ib and UP II, Uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994).Synonyms: UP3a, UPIII, UPK3, Uroplakin III

ID gène: 7380

NCBI Accession: NP_001161046

UniProt: O75631