Keratin 3 anticorps

N° du produit ABIN112318

Cet anticorps Souris Monoclonal détecte spécifiquement Keratin 3 dans WB et IHC (fro). Il présente une réactivité envers Humain, Lapin et Boeuf (Vache).

Aperçu rapide pour Keratin 3 anticorps (ABIN112318)

Antigène: Keratin 3 (KRT3)

Reactivité: Humain, Lapin, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AE5

Détail du produit anti-Keratin 3 anticorps

Specificité: Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.

Réactivité croisée (Details): Species reactivity (tested):Human, Rabbit and Bovine.

Purification: Ion Exchange Chromatography.

Immunogène: Human epithelial keratin

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Keratin 3

Antigène: Keratin 3 (KRT3)

Autre désignation: Cytokeratin 3

Sujet: Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3

ID gène: 3850

NCBI Accession: NP_476429

UniProt: P12035