KRT4 anticorps

N° du produit ABIN112320

Détail du produit anti-KRT4 anticorps

Antigène: KRT4 (Keratin 4 (KRT4))

Reactivité: Humain, Chien, Cat, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT4 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This Monoclonal antibody 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.

Réactivité croisée (Details): Species reactivity (tested):Human, Canine, Feline, Zebrafish.

Purification: Purified

Immunogène: 6B10 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.

Clone: 6B10

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry. Immunohistochemistry on Frozen and Tissues (1/25-1/200) using with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent. Immunoblotting (1/100-1/1000). Flow Cytometry (1/25-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-KRT4 (ABIN112320)

Liu, Li, Tan, Beuerman: "The eyelid margin: a transitional zone for 2 epithelial phenotypes." dans: Archives of ophthalmology (Chicago, Ill. : 1960), Vol. 125, Issue 4, pp. 523-32, (2007) (PubMed).

Détails sur KRT4

Antigène: KRT4 (Keratin 4 (KRT4))

Autre désignation: Cytokeratin 4 (KRT4 Produits)

Synonymes: anticorps cb59, anticorps etID20321.6, anticorps krt8, anticorps wu:fa91a11, anticorps wu:fd19e09, anticorps wu:fl23c09, anticorps zf-K8, anticorps CK-4, anticorps CK4, anticorps CYK4, anticorps K4, anticorps Kb4, anticorps AW108092, anticorps Krt-2.4, anticorps Krt2-4, anticorps keratin 4, anticorps krt4, anticorps KRT4, anticorps Krt4

Sujet: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK4, CYK4, Cytokeratin-4, K4, KRT4, Keratin, Keratin-4, type II cytoskeletal 4

ID gène: 9606

UniProt: P19013