Cytokeratin 13 anticorps

N° du produit ABIN112324

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 13 dans WB, IF, IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Humain, Rat et Boeuf (Vache).

Aperçu rapide pour Cytokeratin 13 anticorps (ABIN112324)

Antigène: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Reactivité: Humain, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 13 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ks13-1

Détail du produit anti-Cytokeratin 13 anticorps

Specificité: Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13, with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine and Rat.

Purification: Protein A affinity chromatography

Immunogène: Keratin K13 of Mr 54.000 purified from human esophagus.

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: 1: 10, protease pretreatmentimproves the performance of this excellent marker in immunohisto-chemistry: 1/10 withPBS, pH 7.4. Western Blot. Incubation time: 1h at RT, extended with parafin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 1 mL dist. water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Cytokeratin 13

Antigène: Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))

Autre désignation: Cytokeratin 13

Sujet: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13

ID gène: 3860

NCBI Accession: NP_002265

UniProt: P13646