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Cytokeratin 7 anticorps

L’anticorps Souris Monoclonal anti-Cytokeratin 7 a été validé pour WB, IF, FACS, IHC (p) et IHC (fro). Il convient pour détecter Cytokeratin 7 dans des échantillons de Humain, Rat, Souris, Chien, Hamster, Cat, Poisson zèbre (Danio rerio) et Chévre.
N° du produit ABIN112349

Aperçu rapide pour Cytokeratin 7 anticorps (ABIN112349)

Antigène

Voir toutes Cytokeratin 7 (KRT7) Anticorps
Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivité

  • 227
  • 74
  • 61
  • 7
  • 5
  • 5
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Rat, Souris, Chien, Hamster, Cat, Poisson zèbre (Danio rerio), Chévre

Hôte

  • 130
  • 108
  • 1
Souris

Clonalité

  • 139
  • 99
  • 1
Monoclonal

Conjugué

  • 99
  • 22
  • 16
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Cet anticorp Cytokeratin 7 est non-conjugé

Application

  • 147
  • 110
  • 94
  • 93
  • 39
  • 31
  • 28
  • 28
  • 27
  • 26
  • 19
  • 17
  • 12
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

RCK105
  • Specificité

    RCK105 reacts exclusively with Cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma.

    Purification

    Purified

    Immunogène

    Cytokeratins from the Human bladder carcinoma cell line T24.

    Isotype

    IgG1
  • Indications d'application

    Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on FrozenSections and Flow Cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100- 1/1000 for Immunoblotting applications.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS with 0.09 % Sodium Azide as preservative

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.
  • Antigène

    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

    Autre désignation

    Cytokeratin 7

    Sujet

    Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

    ID gène

    3855

    NCBI Accession

    NP_005547

    UniProt

    P08729
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