NEFM anticorps

N° du produit ABIN112357

Cet anticorps Souris Monoclonal détecte spécifiquement NEFM dans WB, IF, IHC (p) et IHC (fro). Il présente une réactivité envers Humain, Rat, Singe, Hamster et Xenopus laevis.

Aperçu rapide pour NEFM anticorps (ABIN112357)

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivité: Humain, Rat, Singe, Hamster, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFM est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF403

Détail du produit anti-NEFM anticorps

Specificité: RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.

Purification: Purified

Immunogène: Neurofilament preparation of calf brain tissue.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissues. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent.1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur NEFM

Antigène: NEFM (Neurofilament Triplet M Protein (NEFM))

Autre désignation: Neurofilament M (160 kD)

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide

ID gène: 4741

NCBI Accession: NP_005373

UniProt: P07197

Pathways: Brown Fat Cell Differentiation