NEFH anticorps (phosphospecific)

N° du produit ABIN112359

Cet anticorps anti-NEFH est un anticorps Souris Monoclonal détectant NEFH dans WB, IHC (p) et IHC (fro). Adapté pour Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin, Cobaye, Chien, Mouton et Xenopus laevis.

Aperçu rapide pour NEFH anticorps (phosphospecific) (ABIN112359)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Lapin, Cobaye, Chien, Mouton, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF404

Détail du produit anti-NEFH anticorps

Épitope: phosphospecific

Specificité: RNF404 reacts exclusively with the phosphorylated isoform of the 200 kDa neurofilament protein. Reacts with Bovine, Canine, Chicken, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Sheep, Xenopus.

Purification: Purified

Immunogène: Neurofilament preparation of calf brain tissue.

Isotype: IgG2a

Information d'application

Indications d'application: RNF404 is suitable for Immunoblotting and Immunohistochemistry on frozen tissues. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: Neurofilament H (200 kD)

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide

ID gène: 4744

NCBI Accession: NP_066554

UniProt: P12036