NEFH anticorps

N° du produit ABIN112360

Détail du produit anti-NEFH anticorps

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Cobaye, Lapin, Chien, Mouton, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: RNF402 reacts with both the phosphorylated and non-phosphorylated isoforms of the 200 kDa Neurofilament protein. Reacts with Human, Rat, Rabbit, Dog, Hamster, Chicken, Mouse, Cow, Monkey, Sheep, Guinea Pig and Xenopus.

Purification: Purified

Immunogène: Neurofilament preparation of calf brain tissue.

Clone: RNF402

Isotype: IgM

Information d'application

Indications d'application: ImmunoblottingImmunohistochemistry on Frozen and Paraffin Embedded Tissues. Recommended Dilutions: 1/10-1/100 for Immunohistochemistry with avidin biotinylatedHorseradish Peroxidase complex (ABC) as detection reagent, and 1/25-1/250 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: Neurofilament H (200 kD) (NEFH Produits)

Synonymes: anticorps NF-H, anticorps NF200, anticorps Nfh, anticorps mKIAA0845, anticorps NFH, anticorps NEFH, anticorps neurofilament heavy polypeptide, anticorps neurofilament, heavy polypeptide, anticorps neurofilament heavy, anticorps MCYG_06231, anticorps Nefh, anticorps NEFH

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide

ID gène: 4744

NCBI Accession: NP_066554

UniProt: P12036