OB Cadherin anticorps (Extracellular Domain)

N° du produit ABIN112366

Cet anticorps Souris Monoclonal détecte spécifiquement OB Cadherin dans WB, IF et IHC (fro). Il présente une réactivité avec des échantillons de Humain et Rat.

Aperçu rapide pour OB Cadherin anticorps (Extracellular Domain) (ABIN112366)

Antigène: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp OB Cadherin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 16A

Détail du produit anti-OB Cadherin anticorps

Épitope: Extracellular Domain

Specificité: This antibody recognizes the extracellular domain of Cadherin-11.

Purification: Affinity Chromatography

Immunogène: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Mouse immunized with affinity purified extracellular domain of Human Cadherin-11-GST fusion protein.

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunoblotting, Immunocytochemistry and Immunohistochemistry on Frozensections when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Recommended Dilutions: 1/25-1/50 for Immunohistochemistry with Avidin-biotinylatedHorseradish Peroxidase Complex (ABC) as detection reagent.1/25-1/250 for Immunoblotting applications.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the original vial at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur OB Cadherin

Antigène: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Autre désignation: Cadherin-11

Sujet: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.Synonyms: CDH11, OB Cadherin, OSF-4, Osteoblast cadherin

ID gène: 1009

NCBI Accession: NP_001788

UniProt: P55287