CTNNA3 anticorps (N-Term)

N° du produit ABIN112367

L’anticorps Souris Monoclonal anti-CTNNA3 a été validé pour WB, IF, IP, IHC (p), IHC (fro) et FACS. Il convient pour détecter CTNNA3 dans des échantillons de Humain.

Aperçu rapide pour CTNNA3 anticorps (N-Term) (ABIN112367)

Antigène: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CTNNA3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: 892_24D2S

Détail du produit anti-CTNNA3 anticorps

Épitope: AA 164-177, N-Term

Specificité: The 892_24D2S antibody against αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis. This 892_24D2S antibody reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.

Purification: Purified

Immunogène: Synthetic peptide corresponding to amino acids 164-177 of the human alphaT-catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.

Isotype: IgG2a

Information d'application

Indications d'application: Immunofluorescence: aceton-fixed cells. Immunocytochemistry: methanol and paraformaldehyde-fixed cells, followed bypermeabilisation in 0.2 % Triton-X 100 for 2 min. Immunohistochemistry1: 1/25-1/200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent,Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Détails sur CTNNA3

Antigène: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Autre désignation: Catenin alpha-3 / CTNNA3

Sujet: Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57 % . In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation.Synonyms: Alpha Catenin 3, Alpha T-catenin, Cadherin-associated protein

ID gène: 29119

NCBI Accession: NP_001120856

UniProt: Q9UI47