Plakophilin 3 anticorps (N-Term)

N° du produit ABIN112377

Cet anticorps anti-Plakophilin 3 est un anticorps Souris Monoclonal détectant Plakophilin 3 dans WB, IP, IHC (fro) et IF. Adapté pour Humain, Souris, Chien et Porc.

Aperçu rapide pour Plakophilin 3 anticorps (N-Term) (ABIN112377)

Antigène: Plakophilin 3 (PKP3)

Reactivité: Humain, Souris, Chien, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Plakophilin 3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: 12B11F8

Détail du produit anti-Plakophilin 3 anticorps

Épitope: AA 779-793, N-Term

Specificité: This antibody reacts with an epitope located between residues 779-793 in Human Plakophilin-3.

Purification: Purified

Immunogène: Synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CKLHRDFAKGYRKED).

Isotype: IgG2a

Information d'application

Indications d'application: E612B11F8 is suitable for Immunocytochemistry (methanol-fixed cells),Immunohistochemistry on frozen tissues, Immunoprecipitation and Western blotdetection. For optimal results, fixed cells or tissues should be treated with 0.2 % Triton-X 100 for 5 min. prior to incubation with primary antibody. Recommended dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Détails sur Plakophilin 3

Antigène: Plakophilin 3 (PKP3)

Autre désignation: Plakophilin 3 / PKP3

Sujet: Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3

ID gène: 11187

NCBI Accession: NP_009114

UniProt: Q9Y446