Selectin E/CD62e anticorps

N° du produit ABIN112379

Détail du produit anti-Selectin E/CD62e anticorps

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: Purified

Immunogène: LPS-activated mlEND1 cells expressing E-selectin at their cell surface

Clone: UZ4

Isotype: IgM

Information d'application

Indications d'application: UZ4 is a functional antibody in that it inhibits leukocyte adhesion to both murine andhuman activated endothelial cells. For in vivo application in mice 20 g of purified Ig perinjection is recommended. UZ4 is also suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry(dilution 1: 500). It is not optimal for immunohistochemistry, to analyze mouse E-selectin protein in frozensections, please refer to the anti E-selectin monoclonal antibodies UZ5 (ABIN112380), UZ6(ABIN112381), and UZ7 (ABIN112382).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E / E-Selectin (SELE Produits)

Synonymes: anticorps SELE, anticorps CD62E, anticorps ELAM, anticorps ELAM1, anticorps ESEL, anticorps LECAM2, anticorps E-selectin, anticorps Elam, anticorps selectin E, anticorps selectin, endothelial cell, anticorps SELE, anticorps Sele

Sujet: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF-alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

ID gène: 9606

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling