Reticulon 1 anticorps

N° du produit ABIN112385

Détail du produit anti-Reticulon 1 anticorps

Antigène: Reticulon 1 (RTN1)

Reactivité: Humain, Souris, Rat, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Reticulon 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificité: MON-162 exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues.

Purification: Purified

Immunogène: Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

Clone: MON162

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunocytochemistry, Immunohistochemistry on frozen and paraffinembedded tissue, and Immunoblotting. Recommended dilutions: 1/100-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized antibody at 2-8 °C. Aliquot and store reconstituted antibody at -20 °C.

Détails sur Reticulon 1

Antigène: Reticulon 1 (RTN1)

Autre désignation: Reticulon 1 / RTN1 (RTN1 Produits)

Synonymes: anticorps NSP, anticorps 0710005K15Rik, anticorps 4930441F12Rik, anticorps Nsp, anticorps R75279, anticorps Rtn1-a, anticorps Rtn1-b, anticorps Rtn1-c, anticorps reticulon-1, anticorps RTN1, anticorps RTN1-Cw, anticorps rtn1, anticorps XRTN1-A, anticorps XRTN1-C.1, anticorps xrtn1, anticorps xrtn1-c, anticorps RTN1.2, anticorps Rtn1-C.2, anticorps rtn1-a, anticorps rtn1b, anticorps xRTN1, anticorps RTN1.1, anticorps rtn1a, anticorps xrtn1-c.1, anticorps rtn1l, anticorps wu:fa21b09, anticorps wu:fj35h01, anticorps reticulon 1, anticorps reticulon-1, anticorps reticulon 1 S homeolog, anticorps reticulon 1 L homeolog, anticorps reticulon 1b, anticorps RTN1, anticorps Rtn1, anticorps rtn1, anticorps Rtnl1, anticorps LOC100580843, anticorps rtn1.S, anticorps rtn1.L, anticorps LOC100339591, anticorps rtn1b

Sujet: Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non- SCLC (cell cultures). The epitope of MON-162 is located between amino acid residues 338-422 of Reticulon-1A.Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1

ID gène: 6252

NCBI Accession: NP_066959

UniProt: Q16799