Cardiotin anticorps

N° du produit ABIN112391

L’anticorps Souris Monoclonal anti- a été validé pour WB, IHC (fro) et IHC (p). Il convient pour détecter dans des échantillons de Humain, Porc, Cat, Chien, Chévre, Hamster, Singe, Souris, Rat, Xenopus laevis et Poisson zèbre (Danio rerio).

Aperçu rapide pour Cardiotin anticorps (ABIN112391)

Antigène: Cardiotin

Reactivité: Humain, Porc, Cat, Chien, Chévre, Hamster, Singe, Souris, Rat, Xenopus laevis, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R2G

Détail du produit

Specificité: R2G recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. This antibody reacts with Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish tissues.

Purification: Purified

Immunogène: Total protein extract of Chicken gizzard.

Isotype: IgM

Information d'application

Indications d'application: This antibody is suitable for Immunohistochemistry on frozen and paraffin-embeddedtissue and Immunoblotting. Recommended dilutions: 1/25-1/100 for immunohistochemistry with avidinbiotinylatedhorseradish peroxidase complex (ABC)as detection reagent.1/25-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.

Détail du antigène

Antigène: Cardiotin

Sujet: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.