Cardiotin anticorps

N° du produit ABIN112393

Détail du produit

Antigène: Cardiotin

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody SR-3 reacts with Cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. In immunoblotting assays SR-3 recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits.

Réactivité croisée (Details): Species reactivity (tested):Human and Porcine.

Purification: Purified

Immunogène: 100 kDa cardiotin subunit.

Clone: SR-3

Isotype: IgM

Information d'application

Indications d'application: Suitable for Immunohistochemistry on frozen sections and Immunoblotting. Recommended dilutions: 1/100-1/200 for Immunohistochemistry with avidinbiotinylated horseradish peroxidasecomplex (ABC) as detection reagent.1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilized at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Détail du antigène

Antigène: Cardiotin

Sujet: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.