CHRM3 anticorps (AA 580-589)

N° du produit ABIN112905

Cet anticorps anti-CHRM3 Polyclonal Lapin (ABIN112905) détecte spécifiquement CHRM3 dans WB et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour CHRM3 anticorps (AA 580-589) (ABIN112905)

Antigène: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHRM3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-CHRM3 anticorps

Épitope: AA 580-589

Specificité: Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.

Immunogène: Synthetic peptide M3 (580-589) covalently attached to a carrier protein.

Information d'application

Indications d'application: Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.

Agent conservateur: Azide free

Précaution d'utilisation: Do Not Use Sodium Azide as preservative.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur CHRM3

Antigène: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Autre désignation: Muscarinic Acetylcholine Receptor M3

Sujet: This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3

ID gène: 1131

NCBI Accession: NP_000731

UniProt: P20309

Pathways: Synaptic Membrane