Collagen Type V anticorps

N° du produit ABIN113098

Cet anticorps Lapin Polyclonal détecte spécifiquement Collagen Type V dans EIA et IF. Il présente une réactivité envers Rat.

Aperçu rapide pour Collagen Type V anticorps (ABIN113098)

Antigène: Collagen Type V (COL5) (Collagen, Type V (COL5))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type V est non-conjugé

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Détail du produit anti-Collagen Type V anticorps

Specificité: Cross-Reactivity (% at 1:10 RIA dilution) Rat Collagen Type V 100 % Rat Collagen Types I, III < 0.1 % Rat Elastin < 0.1 %

Purification: Ion exchange chromatography (DEAE-Trisacryl).

Immunogène: Collagen type V extracted and purified from rat skin.

Information d'application

Indications d'application: Suitable for : ELISA (dilution >1: 100-1: 200). IFA with fluorescein anti-rabbit IgG conjugate (didilution at >1: 40 on frozen rat tissuesections). Immunostaining of extra or intracellular components in light microscopy. Quantitation of Collagen Type V in biological fluids or in culture supernatants. (SP)RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Reconstitute with 0.5 mL deionized water.

Précaution d'utilisation: Do Not Add Sodium Azide

Conseil sur la manipulation: . Avoid cycles of freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20 °C or below.

Détails sur Collagen Type V

Antigène: Collagen Type V (COL5) (Collagen, Type V (COL5))

Autre désignation: Collagen Type V

Sujet: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. Type V Collagen is a member of group I Collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V Collagen binds to DNA, heparan sulfate, Thrombospondin, Heparin, and Insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI Collagen and it is possible that the Collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.Synonyms: COL5A1

ID gène: 85490

NCBI Accession: NP_604447

UniProt: Q9JI03