NPY1R anticorps (AA 365-378)
Aperçu rapide pour NPY1R anticorps (AA 365-378) (ABIN113620)
Antigène
Voir toutes NPY1R AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 365-378
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Specificité
- This antibody reacts with Neuropeptide Y Receptor 1.
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Purification
- Ammonium Sulphate Fractionation
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Immunogène
- Synthetic peptide corresponding to amino acids 365-378 of human NPY1R (KQASPVAFKKINNN) conjugated to KLH.
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Indications d'application
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ELISA. Western blot (1/250 as a guide). Immunohistochemistry on Paraffin Sections (1/1,000-1/4,000): See below for protocol.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protocole
- Protocol: - Samples: Bouin's fixed paraffin sections, 5 µm, from rat brain. - 5 minutes microwave pre-treatment, citrate buffer, pH 6. 0. - Peroxidase blocking (15 minutes), normal rabbit serum (30 minutes),
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 5.0 mg/mL
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Buffer
- PBS, pH 7.2, containing 0.09 % Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- Do Not Add Sodium Azide
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- NPY1R (Neuropeptide Y Receptor 1 (NPY1R))
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Autre désignation
- Neuropeptide Y Receptor 1
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Sujet
- Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily.Synonyms: NPYR, NPYY1, Neuropeptide Y receptor 1
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ID gène
- 4886
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NCBI Accession
- NP_000900
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UniProt
- P25929
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Pathways
- Feeding Behaviour
Antigène
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