Fibronectin anticorps

N° du produit ABIN113981

L’anticorps Lapin Polyclonal anti-Fibronectin a été validé pour IHC (p), EIA, IHC (fro) et RIA. Il convient pour détecter Fibronectin dans des échantillons de Souris.

Aperçu rapide pour Fibronectin anticorps (ABIN113981)

Antigène: Fibronectin

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Détail du produit anti-Fibronectin anticorps

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogène: Purified fibronectin from Murine plasma.

Information d'application

Indications d'application: This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P)(1/500) After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8 °C over night. Also suitable for ELISA (1/200) and RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water, dilute further with appropriate antibody diluent.

Concentration: 1.0 mg/mL

Buffer: PBS without preservative or stabilizer.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

ID gène: 10090

UniProt: P11276