Collagen Type I anticorps
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- Antigène Voir toutes Collagen Type I (COL1) Anticorps
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Collagen Type I est non-conjugé
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Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)
- Specificité
- Human Collagen type I: 100 % Collagen type II and IV: < 0.1 % Collagen type III and V: < 1.0 % Human Fibronectin: < 0.1 %.
- Réactivité croisée (Details)
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Species reactivity (expected):Cattle (< 1.0 %), Rat and Chicken (< 0.1 %).
Species reactivity (tested):Human. - Purification
- Affinity Chromatography
- Immunogène
- Purified Collagen type I from Human skin.
- Top Product
- Discover our top product COL1 Anticorps primaire
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- Indications d'application
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RIA. ELISA: >/= 1/200 (OD >/= 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Human skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 0.5 mL distilled water.
- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered solution, no BSA and preservative added.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- Autre désignation
- Collagen Type I (COL1 Produits)
- Synonymes
- anticorps collagen type III alpha 1 chain, anticorps COL3A1
- Sujet
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- ID gène
- 1277
- NCBI Accession
- NP_000079
- UniProt
- P02452
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