CD90 anticorps (Biotin)

N° du produit ABIN114250

Détail du produit anti-CD90 anticorps (Biotin)

Antigène: CD90 (THY1)

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD90 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody detects CD90 (Thy 1.2). It reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (i.e. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype (i.e. AKR/J, B6.PL (74 NS).

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Protein G Chromatography.

Immunogène: CBA/J Donor: AKR/J Spleen

Clone: 5a-8

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry (protocol see below). Appropriate control samples should always be included in any labelling studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2-0. 5 µg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1/500 dilution. 9. Incubate tubes at 4°C for 30-60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: CBA/JCell Concentration : 1x10e6 cells per testsAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2b,kCell Source: Percentage of cells stained above control: Thymus: 97. 8%Spleen: 35. 4%

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C.

Détails sur CD90

Antigène: CD90 (THY1)

Autre désignation: CD90 (THY1 Produits)

Synonymes: anticorps CD90, anticorps CD7, anticorps T25, anticorps Thy-1, anticorps Thy-1.2, anticorps Thy1.1, anticorps Thy1.2, anticorps Thy-1 cell surface antigen, anticorps thymus cell antigen 1, theta, anticorps THY1, anticorps Thy1

Sujet: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

ID gène: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization