This mAb is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate or eliminate cells bearing H-2Dd (H-2.4) antigen from the appropriate strains of mice.
Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocole
RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OFH-2Dd BEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viable
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, no preservative.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
H-2Dd
(MHC Class I H-2Dd)
Autre désignation
MHC Class I H-2 Dd
Sujet
Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1