Cet anticorps anti-MHC Class I (H-2Kb/H-2Db) Monoclonal Souris conjugué à Biotin (Clone 5041-16-1) (ABIN114294) détecte spécifiquement MHC Class I (H-2Kb/H-2Db) dans FACS.
L’anticorps est réactif avec des échantillons de Souris.
Flow Cytometry (see Protocol below). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocole
FLOW CYTOMETRY ANALYSISMethod: 1. Prepare a cell suspension in media A. For spleen cell preparations, deplete the red blood
Restrictions
For Research Use only
Concentration
0.1 mg IgG /ml
Buffer
PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.