MHC Class II I-Abd anticorps

N° du produit ABIN114309

Cet anticorps anti-MHC Class II I-Abd Monoclonal Souris (Clone 28-16-8S) (ABIN114309) détecte spécifiquement MHC Class II I-Abd dans CyTox. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour MHC Class II I-Abd anticorps (ABIN114309)

Antigène: MHC Class II I-Abd

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Cytotoxicity Test (CyTox)

Clone: 28-16-8S

Détail du produit

Purification: Affinity chromatography

Immunogène: C3H.SW spleen Donor: C3H Fusion Partner: SP2/0Ag.14

Isotype: IgM

Information d'application

Indications d'application: Flow Cytometry. Can be used to quantitate or eliminate cells bearing the I-Ab antigen from the appropriatestrains of mice.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1. 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 640 and mix.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS and contains 0.02 % sodium azide (NaN3) as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: MHC Class II I-Abd