MHC Class II I-Ap anticorps

N° du produit ABIN114311

L’anticorps anti-MHC Class II I-Ap Monoclonal Souris est utilisé pour la détection de MHC Class II I-Ap dans des échantillons de Souris. Il a été validé pour FACS et CyTox.

Aperçu rapide pour MHC Class II I-Ap anticorps (ABIN114311)

Antigène: MHC Class II I-Ap

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox)

Clone: 7-16-17

Détail du produit

Purification: Ig Fraction

Immunogène: Recipient: BALB/c Donor: B10.P Fusion Partner: Spleen from immunized recipient fused with myeloma SP2/0

Isotype: IgG2a

Information d'application

Indications d'application: Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF I-A ANTIGEN BEARINGCELLS: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium®1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte-M®2 density cell separation medium. After washing, adjustthe cell concentration to 1 x 10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 40 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 40 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 1.0 mL of distilled water.

Buffer: PBS

Précaution d'utilisation: Use of sodium azide as a preservative will substantially inhibit the enUse of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: MHC Class II I-Ap