MHC Class II I-Ap anticorps (Biotin)

N° du produit ABIN114312

Cet anticorps anti-MHC Class II I-Ap Monoclonal Souris conjugué à Biotin (Clone 7-16-17) (ABIN114312) détecte spécifiquement MHC Class II I-Ap dans FACS. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour MHC Class II I-Ap anticorps (Biotin) (ABIN114312)

Antigène: MHC Class II I-Ap

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS)

Clone: 7-16-17

Détail du produit

Purification: Protein G Chromatography

Immunogène: B10.P Donor: BALB/c Fusion Partner: SP2/0

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 - 0. 5 µg* of this Ab per 10e6 cells.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: MHC Class II I-Ap