NEFL anticorps

N° du produit ABIN114492

Cet anticorps anti-NEFL est un anticorps Souris Monoclonal détectant NEFL dans WB, IHC (p) et IHC (fro). Adapté pour Humain, Rat, Souris, Opossum, Cat et Lapin.

Aperçu rapide pour NEFL anticorps (ABIN114492)

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivité: Humain, Rat, Souris, Opossum, Cat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2F11

Détail du produit anti-NEFL anticorps

Specificité: 2F11 reacts exclusively with the phosphorylated isoform of the 70 kDa neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.

Réactivité croisée (Details): Also reacts to: Neurofilament H

Purification: Purified

Immunogène: Human neurofilament preparation

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen and paraffin embedded sections: This antibody may bediluted to a titer of 1: 50-1: 200 in an ABC method. Immunoblotting: 1: 200 -1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Détails sur NEFL

Antigène: NEFL (Neurofilament, Light Polypeptide (NEFL))

Autre désignation: Neurofilament L (68kDa)

Sujet: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiationSynonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L

Poids moléculaire: 68 kDa and 208 kDa (phosphorylated forms of these subunits).

ID gène: 4747

NCBI Accession: NP_006149

UniProt: P07196