NEFL anticorps
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- Antigène Voir toutes NEFL Anticorps
- NEFL (Neurofilament, Light Polypeptide (NEFL))
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Reactivité
- Humain, Rat, Souris, Opossum, Cat, Lapin
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NEFL est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- 2F11 reacts exclusively with the phosphorylated isoform of the 70 kDa neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.
- Réactivité croisée (Details)
- Also reacts to: Neurofilament H
- Purification
- Purified
- Immunogène
- Human neurofilament preparation
- Clone
- 2F11
- Isotype
- IgG1
- Top Product
- Discover our top product NEFL Anticorps primaire
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- Indications d'application
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Immunohistochemistry on frozen and paraffin embedded sections: This antibody may bediluted to a titer of 1: 50-1: 200 in an ABC method. Immunoblotting: 1: 200 -1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
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- Antigène
- NEFL (Neurofilament, Light Polypeptide (NEFL))
- Autre désignation
- Neurofilament L (68kDa) (NEFL Produits)
- Synonymes
- anticorps NEFL, anticorps nefl, anticorps zgc:136626, anticorps AI847934, anticorps CMT2E, anticorps NF-L, anticorps NF68, anticorps Nfl, anticorps CMT1F, anticorps NFL, anticorps XNF-L, anticorps neurofilament light, anticorps neurofilament, light polypeptide, anticorps neurofilament, light polypeptide b, anticorps neurofilament, light L homeolog, anticorps NEFL, anticorps neflb, anticorps Nefl, anticorps nefl.L
- Sujet
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiationSynonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L
- Poids moléculaire
- 68 kDa and 208 kDa (phosphorylated forms of these subunits).
- ID gène
- 4747
- NCBI Accession
- NP_006149
- UniProt
- P07196
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