Myeloperoxidase anticorps

N° du produit ABIN115370

Détail du produit anti-Myeloperoxidase anticorps

Antigène: Myeloperoxidase (MPO)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myeloperoxidase est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Purified

Immunogène: Myeloperoxidase isolated from human granulocytes.

Information d'application

Indications d'application: Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/100 in an ABC method (30 minutes at room temperature). Requires hightemperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one mounth or (in aliquots) at -20 °C for longer.

Détails sur Myeloperoxidase

Antigène: Myeloperoxidase (MPO)

Autre désignation: Myeloperoxidase (MPO Produits)

Synonymes: anticorps mKIAA4033, anticorps MPO, anticorps LOC100335032, anticorps POX2', anticorps XPOX2', anticorps mpo, anticorps mpo-A, anticorps pmr-1, anticorps pmr1, anticorps pox2, anticorps xpox2, anticorps myeloperoxidase, anticorps myeloperoxidase L homeolog, anticorps MPO, anticorps Mpo, anticorps LOC100335032, anticorps mpo.L

Sujet: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5 % . MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.Synonyms: MPO

ID gène: 9606

UniProt: P05164

Pathways: Chromatin Binding