HBxAg anticorps

N° du produit ABIN115505

Détail du produit anti-HBxAg anticorps

Antigène: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Reactivité: Hepatitis B Virus (HBV)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HBxAg est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Séquence: Total 154 AA: 153 AA of recombinan t HBx protein and one extra AA, N-terminal methionin (underlined ). MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNF FTSA

Purification: Immunoaffinity Chromatography on a column with immobilized recombinant Hepatitis B Protein X

Immunogène: Recombinant Hepatitis B Protein X from E. coli.

Information d'application

Indications d'application: ELISA. (Titer is defined by Indirect ELISA, it is: >1: 100,000 for antibody concentration 1 mg/mL, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of thetitration curve). Western blotting. Immunoprecipitaion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Buffer: 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-HBxAg (ABIN115505)

Gouas, Shi, Hautefeuille, Ortiz-Cuaran, Legros, Szymanska, Galy, Egevad, Abedi-Ardekani, Wiman, Hantz, Caron de Fromentel, Chemin, Hainaut: "Effects of the TP53 p.R249S mutant on proliferation and clonogenic properties in human hepatocellular carcinoma cell lines: interaction with hepatitis B virus X protein." dans: Carcinogenesis, Vol. 31, Issue 8, pp. 1475-82, (2010) (PubMed).

Zhang, Yang, Zhang, Xu, Liao, Song, Wang: "Induction of hepatic enzymes and oxidative stress in Chinese rare minnow (Gobiocypris rarus) exposed to waterborne hexabromocyclododecane (HBCDD)." dans: Aquatic toxicology (Amsterdam, Netherlands), Vol. 86, Issue 1, pp. 4-11, (2008) (PubMed).

Détails sur HBxAg

Antigène: HBxAg (Hepatitis B Virus X Antigen (HBxAg))

Autre désignation: Hepatitis B X Protein / HBx (HBxAg Produits)

Classe de substances: Viral Protein

Sujet: The recombinant Hepatitis B Protein X is 100 % homologous with the natural Hepatitis B Protein X. Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.Synonyms: HBV Protein X

Poids moléculaire: 17 kDa