PLGF anticorps (N-Term)

N° du produit ABIN115668

Cet anticorps anti-PLGF est un anticorps Lapin Polyclonal détectant PLGF dans WB, IF et EIA. Adapté pour Humain.

Aperçu rapide pour PLGF anticorps (N-Term) (ABIN115668)

Antigène: PLGF (PGF) (Placenta Growth Factor (PGF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLGF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-PLGF anticorps

Épitope: AA 19-38, N-Term

Specificité: The antibody will react with all Human PlGF isoforms.

Purification: Affinity Chromatography on Protein A

Immunogène: N-terminal 20 amino acid peptide of native Human PIGF (Leu19 - Val38) produced in Insect Cells.

Information d'application

Indications d'application: Western Blot: 10 μg/mL, it will detect 25-50 ng/lane of recombinant human PlGF-1 andPlGF-2 under reducing conditions. Dimers are detected at higher protein amounts/lane. ELISA: 1-5 μg/mL, allows the detection of 1.0-2.5 ng/well of recombinant human PlGF-1 andPlGF-2. Immunocytochemistry: 5-25 μg/mL, allows the detection of PlGF protein in different humantissue including placenta and brain tumours.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur PLGF

Antigène: PLGF (PGF) (Placenta Growth Factor (PGF))

Autre désignation: Placenta Growth Factor / PGF

Sujet: Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors that share a conserved pattern of eight cysteines. Alternate splicing results in at least three human mature PlGF forms containing 131 (PlGF1), 152 (PlGF2), and 203 (PlGF3) amino acids (aa) respectively. Only PlGF2 contains a highly basic heparinbinding 21 aa insert at the C-terminus. In the mouse, only one P lGF that is the equivalent of human PlGF2 has been identified. Human PlGF1 shares 56 % , 55 %, 74 % and 95 % aa identity with the appropriate isoform of mouse, rat, canine and equine PlGF. PlGF is mainly found as variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts, keratinocytes and some endothelial cells. Circulating PlGF increases during pregnancy, reaching a peak in mid-gestation, this increase is attenuated in preeclampsia. However, deletion of PlGF in the mouse does not affect development or reproduction. Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia. PlGF binds and signals through VEGF R1/Flt1, but not VEGF R2/Flk-1/KDR, while VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage VEGF/VEGF R1 binding and promote VEGF/VEGF R2mediated angiogenesis. However, PlGF (especially PlGF1) and some forms of VEGF can form dimers that decrease the angiogenic effect of VEGF on VEGF R2. PlGF2, but not PLGF-1, shows heparindependent binding of neuropilin (Npn)-1 and Npn2. PlGF induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing, but also contribute to inflammation in active sickle cell disease and atherosclerosis.Synonyms: PGFL, PLGF, PlGF

ID gène: 5228

NCBI Accession: NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals