CCL16 anticorps
Aperçu rapide pour CCL16 anticorps (ABIN115778)
Antigène
Voir toutes CCL16 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody recognises Human LEC.
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Purification
- Affinity Chromatography on Protein A
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Immunogène
- Highly pure (>98%) recombinant Human LEC
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Isotype
- IgG1
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman LEC (100 ng/mL), a concentration of 1.0-5.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of thisantibody will detect at least 0.03 ng/well of recombinant human LEC when used withbiotinylated antigen affinity purified anti-human LEC as the detection antibody at aconcentration of at least 1 μg/mL. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 1.0 mg/mL.
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Buffer
- PBS without preservatives
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))
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Autre désignation
- NCC4 / CCL16
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Sujet
- LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.Synonyms: C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, ILINCK, LCC-1, LCC1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, SCYA16
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ID gène
- 6360
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NCBI Accession
- NP_004581
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UniProt
- O15467
Antigène
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