IL-10 anticorps (Biotin)

N° du produit ABIN115867

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-10 dans WB et EIA. Il présente une réactivité envers Souris.

Aperçu rapide pour IL-10 anticorps (Biotin) (ABIN115867)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-10 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-10 anticorps (Biotin)

Specificité: Recognizes Murine IL-10.

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogène: Highly pure (>98%) recombinant Mouse IL-10.

Information d'application

Indications d'application: ELISA: To detect mIL-10 by direct ELISA (using 100 ml/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-10. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody can be stored for one year from despatch at -20 °C. Reconstituted antibody is stable for two weeks at 2-8 °C, frozen aliquots are stable for six months at -20 °C.

Date de péremption: 12 months

Détails sur IL-10

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: Interleukin-10 / IL10

Sujet: Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R), a 560 amino acid, 110 kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF

ID gène: 16153

NCBI Accession: NP_034678

UniProt: P18893

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints