CX3CL1 anticorps (Biotin)

N° du produit ABIN116015

Détail du produit anti-CX3CL1 anticorps (Biotin)

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CX3CL1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Human Fractalkine.

Purification: Affinity chromatography

Immunogène: Highly pure (> 98%) recombinant hFractalkine (human Fractalkine)

Information d'application

Indications d'application: ELISA: (Direct): To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFractalkine. (Sandwich): To detect hFractalkine by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Fractalkine (PP1009P) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2, without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Date de péremption: 12 months

Détails sur CX3CL1

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Autre désignation: Fractalkine / CX3CL1 (CX3CL1 Produits)

Synonymes: anticorps ABCD3, anticorps CX3CL1, anticorps DKFZp459K117, anticorps Cx3c, anticorps Scyd1, anticorps ABCD-3, anticorps C3Xkine, anticorps CXC3, anticorps CXC3C, anticorps NTN, anticorps NTT, anticorps SCYD1, anticorps fractalkine, anticorps neurotactin, anticorps AB030188, anticorps AI848747, anticorps CX3C, anticorps Cxc3, anticorps D8Bwg0439e, anticorps ATP binding cassette subfamily D member 3, anticorps C-X3-C motif chemokine ligand 1, anticorps chemokine (C-X3-C motif) ligand 1, anticorps ABCD3, anticorps CX3CL1, anticorps Cx3cl1, anticorps Abcd3

Sujet: CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1

ID gène: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane