CXCL3
Reactivité: Souris
Hôte: Lapin
Polyclonal
FITC
Indications d'application
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0-2.0 μg/mL of this antibody is required. ELISAIndirect: To detect hGRO-gamma by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hGRO-gamma. Sandwich: To detect hGRO-gamma by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human GRO-gamma (PP1014B1 orPP1014B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO-gamma. Western BlotTo detect hGRO-gamma by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 0.7-0.5 μg/mL withan overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with aDAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives
Agent conservateur
Without preservative
Conseil sur la manipulation
This product is photosensitive and should be protected from light
Growth Regulated Protein gamma (GRO gamma) is a cytokine that may play a role in inflammation and affects endothelial cells in an autocrine fashion. It belongs to the intercrine alpha family (small cytokine c-x-c) (chemokine cxc).Synonyms: C-X-C motif chemokine 3, GRO-gamma, GRO3, GROG, Growth-regulated protein gamma, MIP2-beta, Macrophage inflammatory protein 2-beta, SCYB3